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phosphorylated stat2 y690  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphorylated stat2 y690
    Phosphorylated Stat2 Y690, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphorylated+stat2+y690/Phospho-Stat2+(Tyr690)+Rabbit+mAb/pm39593161-138-39-43
    Average 96 stars, based on 186 article reviews
    phosphorylated stat2 y690 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Pteropus vampyrus TRIM40 Is an Interferon-Stimulated Gene That Antagonizes RIG-I-like Receptors
    Article Snippet: Protein samples were run on 4–15% or 7.5% Mini-PROTEAN- or Criterion-TGX Precast Gels (Bio-Rad, Hercules, CA, USA). .. The proteins were then transferred onto methanol-activated Immun-Blot PVDF membrane (Bio-Rad), and the membrane was blocked with 5% Carnation powdered skim milk (Nestle, Vevey, Vaud, Switzerland) in 1× TBS-T (blocking buffer) for 1 h. Primary antibodies were prepared in 2% bovine serum albumin 1× TBS-T with 0.02% sodium azide to the appropriate dilution: anti-FLAG (Sigma) 1:2000, anti-HA (Sigma) 1:2000, anti-His (Sigma) 1:2000, phosphorylated TBK1 S172 (Epitomics, Burlingame, CA, USA) 1:1000, total TBK1 (Novus Biologicals, Centennial, CO, USA) 1:1000, phosphorylated STAT1 Y701 (Cell Signaling, Danvers, MA, USA) 1:1000, total STAT1 (Cell Signaling) 1:1000, phosphorylated STAT2 Y690 (Cell Signaling) 1:1000, total STAT2 (Cell Signaling) 1:1000, anti-ubiquitin (Enzo, Farmingdale, NY, USA) 1:1000, anti-tubulin (Sigma) 1:2000, and anti-actin (Abcam, Cambridge, UK) 1:2000. .. The next day, the blot was washed in 1× TBS-T prior to incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare, Chicago, IL, USA) or goat-anti-mouse (GE health care) for 1 h. The blot was then washed and developed using Pierce ECL Western Blotting Substrate (Thermo Fisher) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher).

    Article Title: Pteropus vampyrus TRIM40 Is an Interferon-Stimulated Gene That Antagonizes RIG-I-like Receptors.
    Article Snippet: Protein samples were run on 4–15% or 7.5% Mini-PROTEAN- or Criterion-TGX Precast Gels (Bio-Rad, Hercules, CA, USA). .. The proteins were then transferred onto methanolactivated Immun-Blot PVDF membrane (Bio-Rad), and the membrane was blocked with 5% Carnation powdered skim milk (Nestle, Vevey, Vaud, Switzerland) in 1× TBS-T (blocking buffer) for 1 h. Primary antibodies were prepared in 2% bovine serum albumin 1× TBS-T with 0.02% sodium azide to the appropriate dilution: anti-FLAG (Sigma) 1:2000, anti-HA (Sigma) 1:2000, anti-His (Sigma) 1:2000, phosphorylated TBK1 S172 (Epitomics, Burlingame, CA, USA) 1:1000, total TBK1 (Novus Biologicals, Centennial, CO, USA) 1:1000, phosphorylated STAT1 Y701 (Cell Signaling, Danvers, MA, USA) 1:1000, total STAT1 (Cell Signaling) 1:1000, phosphorylated STAT2 Y690 (Cell Signaling) 1:1000, total STAT2 (Cell Signaling) 1:1000, anti-ubiquitin (Enzo, Farmingdale, NY, USA) 1:1000, antitubulin (Sigma) 1:2000, and anti-actin (Abcam, Cambridge, UK) 1:2000. .. The next day, the blot was washed in 1× TBS-T prior to incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare, Chicago, IL, USA) or goat-anti-mouse (GE health care) for 1 h. The blot was then washed and developed using Pierce ECL Western Blotting Substrate (Thermo Fisher) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher).

    Blocking Assay:

    Article Title: Pteropus vampyrus TRIM40 Is an Interferon-Stimulated Gene That Antagonizes RIG-I-like Receptors
    Article Snippet: Protein samples were run on 4–15% or 7.5% Mini-PROTEAN- or Criterion-TGX Precast Gels (Bio-Rad, Hercules, CA, USA). .. The proteins were then transferred onto methanol-activated Immun-Blot PVDF membrane (Bio-Rad), and the membrane was blocked with 5% Carnation powdered skim milk (Nestle, Vevey, Vaud, Switzerland) in 1× TBS-T (blocking buffer) for 1 h. Primary antibodies were prepared in 2% bovine serum albumin 1× TBS-T with 0.02% sodium azide to the appropriate dilution: anti-FLAG (Sigma) 1:2000, anti-HA (Sigma) 1:2000, anti-His (Sigma) 1:2000, phosphorylated TBK1 S172 (Epitomics, Burlingame, CA, USA) 1:1000, total TBK1 (Novus Biologicals, Centennial, CO, USA) 1:1000, phosphorylated STAT1 Y701 (Cell Signaling, Danvers, MA, USA) 1:1000, total STAT1 (Cell Signaling) 1:1000, phosphorylated STAT2 Y690 (Cell Signaling) 1:1000, total STAT2 (Cell Signaling) 1:1000, anti-ubiquitin (Enzo, Farmingdale, NY, USA) 1:1000, anti-tubulin (Sigma) 1:2000, and anti-actin (Abcam, Cambridge, UK) 1:2000. .. The next day, the blot was washed in 1× TBS-T prior to incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare, Chicago, IL, USA) or goat-anti-mouse (GE health care) for 1 h. The blot was then washed and developed using Pierce ECL Western Blotting Substrate (Thermo Fisher) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher).

    Article Title: Pteropus vampyrus TRIM40 Is an Interferon-Stimulated Gene That Antagonizes RIG-I-like Receptors.
    Article Snippet: Protein samples were run on 4–15% or 7.5% Mini-PROTEAN- or Criterion-TGX Precast Gels (Bio-Rad, Hercules, CA, USA). .. The proteins were then transferred onto methanolactivated Immun-Blot PVDF membrane (Bio-Rad), and the membrane was blocked with 5% Carnation powdered skim milk (Nestle, Vevey, Vaud, Switzerland) in 1× TBS-T (blocking buffer) for 1 h. Primary antibodies were prepared in 2% bovine serum albumin 1× TBS-T with 0.02% sodium azide to the appropriate dilution: anti-FLAG (Sigma) 1:2000, anti-HA (Sigma) 1:2000, anti-His (Sigma) 1:2000, phosphorylated TBK1 S172 (Epitomics, Burlingame, CA, USA) 1:1000, total TBK1 (Novus Biologicals, Centennial, CO, USA) 1:1000, phosphorylated STAT1 Y701 (Cell Signaling, Danvers, MA, USA) 1:1000, total STAT1 (Cell Signaling) 1:1000, phosphorylated STAT2 Y690 (Cell Signaling) 1:1000, total STAT2 (Cell Signaling) 1:1000, anti-ubiquitin (Enzo, Farmingdale, NY, USA) 1:1000, antitubulin (Sigma) 1:2000, and anti-actin (Abcam, Cambridge, UK) 1:2000. .. The next day, the blot was washed in 1× TBS-T prior to incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare, Chicago, IL, USA) or goat-anti-mouse (GE health care) for 1 h. The blot was then washed and developed using Pierce ECL Western Blotting Substrate (Thermo Fisher) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher).



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